Assembling a Low-volume Biofuel Cell on a Screen-printed Electrode for Glucose Sensing

Becker, J.M. and Lielpetere, A. and Szczesny, J. and Ruff, A. and Conzuelo, F. and Schuhmann, W.

Volume: 34 Pages: 1629-1637
DOI: 10.1002/elan.202200084
Published: 2022

An enzymatic biofuel cell is integrated on a screen-printed electrode as a basis for a self-powered biosensor. A glucose/O2 biofuel cell consisting of a pyrroloquinoline quinone-dependent glucose dehydrogenase embedded within an Os-complex modified redox polymer bioanode to oxidize glucose and a non-limiting bilirubin oxidase-based gas diffusion biocathode in the direct-electron transfer regime for the reduction of O2 showed a glucose-dependent current and power output. For full integration on a single screen-printed electrode, a miniaturized agar salt bridge was introduced between the two bioelectrodes to ensure operation of the assembly in a two-compartment configuration with each electrode operating at optimal conditions. © 2022 The Authors. Electroanalysis published by Wiley-VCH GmbH.

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