Monitoring Potential-Induced DNA Dehybridization Kinetics for Single Nucleotide Polymorphism Detection by using In Situ Surface Enhanced Raman Scattering

Kayran, Y.U. and Cinar, N. and Jambrec, D. and Schuhmann, W.

Volume: Pages:
DOI: 10.1002/celc.201701220
Published: 2018

Changes in temperature, ionic strength or electrical field are generally employed to dehybridize double-stranded DNA (dsDNA). In contrast, we propose potential-induced dsDNA dehybridization to distinguish fully matched target DNA (tDNA) from tDNA with a single nucleotide polymorphism (SNP) by following their dehybridization kinetics through insitu surface enhanced Raman scattering (SERS). The determination of the potential that evokes notable dehybridization of dsDNA without causing any desorption of the immobilized probe DNA (pDNA) from the electrode surface was performed by investigating the desorption kinetics of labelled single-stranded DNA (ssDNA) and dehybridization kinetics of dsDNA with labelled tDNA. This comparatively simple approach allows for SNP detection within minutes. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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