Publications

Effect of ozone stress on the intracellular metabolites from Cobetia marina

Li, J. and Rumancev, C. and Lutze, H.V. and Schmidt, T.C. and Rosenhahn, A. and Schmitz, O.J.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume: 412 Pages: 5853-5861
DOI: 10.1007/s00216-020-02810-6
Published: 2020

Abstract
A GCxGC-MS system was employed with a non-polar × mid-polar column set for the metabolic non-target analysis of Cobetia marina, the model bacteria for marine biofouling. C. marina was treated with ozone to investigate the intracellular metabolic state change under oxidative stress. A minimal inhibitory concentration test was involved to guarantee that the applied ozone dosages were not lethal for the cells. In this study, non-target analyses were performed to identify the metabolites according to the NIST database. As a result, over 170 signals were detected under normal living conditions including 35 potential metabolites. By the comparison of ozone-treated and non-treated samples, five compounds were selected to describe observed trends of signals in the contour plots. Oleic acid exhibited a slight growth by increasing ozone dosage. In contrast, other metabolites such as the amino acid l-proline showed less abundance after ozone treatment, which was more evident once ozone dosage was raised. Thus, this work could provide a hint for searching for up/downregulating factors in such environmental stress conditions for C. marina. [Figure not available: see fulltext.]. © 2020, The Author(s).

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