Controlling DNA/Surface Interactions for Potential Pulse-Assisted Preparation of Multi-Probe DNA Microarrays

Jambrec, D. and Kayran, Y.U. and Schuhmann, W.

Volume: 31 Pages: 1943-1951
DOI: 10.1002/elan.201900233
Published: 2019

The possibility of selectively modifying microarray electrodes with different DNA sequences in a controlled way without the need for local positioning of solutions or local modification of array surfaces is demonstrated. Potential pulse sequences are employed to perform sequential surface modification of a 32-gold-electrode array with two different thiolated DNA capture sequences, surface passivation and regeneration of selected microarray electrodes, all by adjusting the potential intensities of the same potential pulse-assisted method. We achieve reproducible and controlled DNA immobilization together with minimization of false signals originating from unspecific adsorption or undesired co-immobilization. This methodology is not limited to DNA chips and it is potentially suitable for a wide range of applications employing Au−S chemistry. It can be employed in laboratory conditions for localizing different reactive chemistries onto predefined electrodes of an array without the need for complex and expensive apparatus and special conditions. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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