Publications

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells

Dadras, M. and May, C. and Wagner, J.M. and Wallner, C. and Becerikli, M. and Dittfeld, S. and Serschnitzki, B. and Schilde, L. and Guntermann, A. and Sengstock, C. and Köller, M. and Seybold, D. and Geßmann, J. and Schildhauer, T.A. and Lehnhardt, M. and Marcus, K. and Behr, B.

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume: 24 Pages: 11814-11827
DOI: 10.1111/jcmm.15797
Published: 2020

Abstract
Mesenchymal stromal cells are promising candidates for regenerative applications upon treatment of bone defects. Bone marrow-derived stromal cells (BMSCs) are limited by yield and donor morbidity but show superior osteogenic capacity compared to adipose-derived stromal cells (ASCs), which are highly abundant and easy to harvest. The underlying reasons for this difference on a proteomic level have not been studied yet. Human ASCs and BMSCs were characterized by FACS analysis and tri-lineage differentiation, followed by an intraindividual comparative proteomic analysis upon osteogenic differentiation. Results of the proteomic analysis were followed by functional pathway analysis. 29 patients were included with a total of 58 specimen analysed. In these, out of 5148 identified proteins 2095 could be quantified in >80% of samples of both cell types, 427 in >80% of ASCs only and 102 in >80% of BMSCs only. 281 proteins were differentially regulated with a fold change of >1.5 of which 204 were higher abundant in BMSCs and 77 in ASCs. Integrin cell surface interactions were the most overrepresented pathway with 5 integrins being among the proteins with highest fold change. Integrin 11a, a known key protein for osteogenesis, could be identified as strongly up-regulated in BMSC confirmed by Western blotting. The integrin expression profile is one of the key distinctive features of osteogenic differentiated BMSCs and ASCs. Thus, they represent a promising target for modifications of ASCs aiming to improve their osteogenic capacity and approximate them to that of BMSCs. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd

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