A Multiperspective Approach to Solvent Regulation of Enzymatic Activity: HMG-CoA Reductase

Dirkmann, M. and Iglesias-Fernández, J. and Muñoz, V. and Sokkar, P. and Rumancev, C. and von Gundlach, A. and Krenczyk, O. and Vöpel, T. and Nowack, J. and Schroer, M.A. and Ebbinghaus, S. and Herrmann, C. and Rosenhahn, A. and Sanchez-Garcia, E. and Schulz, F.

Volume: 19 Pages: 153-158
DOI: 10.1002/cbic.201700596
Published: 2018

3-Hydroxy-3-methylglutaryl–coenzyme A (HMG-CoA) reductase was investigated in different organic cosolvents by means of kinetic and calorimetric measurements, molecular dynamics simulations, and small-angle X-ray scattering. The combined experimental and theoretical techniques were essential to complement each other's limitations in the investigation of the complex interaction pattern between the enzyme, different solvent types, and concentrations. In this way, the underlying mechanisms for the loss of enzyme activity in different water-miscible solvents could be elucidated. These include direct inhibitory effects onto the active center and structural distortions. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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